Coding
Part:BBa_K4835033:Design
Designed by: Kun Zhang Group: iGEM23_XJU-China (2023-10-11)
The protein-coding gene thrC can be used to translate threonine synthase
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 346
Illegal PstI site found at 1175 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 346
Illegal PstI site found at 1175 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 346
Illegal PstI site found at 1175 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 346
Illegal PstI site found at 1175
Illegal AgeI site found at 430 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 121
Illegal SapI.rc site found at 1318
Design Notes
If you want to use it in a project, you need to be careful not to affect other genes.
Source
Reference Genome: Halomonas campaniensis LS21